MOTS-c is a 16–amino-acid mitochondrial-derived peptide (MDP) that acts as a metabolic regulator, primarily through AMPK activation. Preclinical studies show it enhances insulin sensitivity, promotes fat oxidation, improves exercise capacity, and counters age-related metabolic decline. No clinical trials have been completed in humans to date. This educational protocol presents a once-daily subcutaneous approach with gradual titration.
Reconstitute: Add 3.0 mL bacteriostatic water → ~13.33 mg/mL concentration.
Typical daily range: 200–1,000 mcg once daily (gradual titration over 10 weeks).
Easy measuring: At 13.33 mg/mL, 1 unit = 0.01 mL ≈ 133.3 mcg on a U‑100 insulin syringe.
Storage: Lyophilized: freeze at −20 °C (−4 °F) or below; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 7 days for best potency.
Frequency: Inject once daily subcutaneously. Stay at each dose level for approximately 2 weeks before increasing, and monitor for any adverse reactions.
For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.
Reconstitution Steps
Draw 3.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming.
Gently swirl/roll until dissolved (do not shake vigorously).
Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Use within 7 days for optimal potency[7].